Produce High-quality Fucosylated Chondroitin Sulfate (FCS) with Confidence
With the continuous exploitation of Marine Biomolecules, more and more Marine Carbohydrates are being discovered and used in the food industry, cosmetic industry, and pharmaceutical industry. FCS is a special type of Marine Animal Polysaccharide. FCS has a similar backbone to Chondroitin Sulfate (CS), consisting of repeating disaccharide units composed of sulfated modified glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc), as well as sulfated or non-sulfated L-fucose (L-Fuc) branching components. FCS possesses different biological activities due to the different patterns of main chain sulfation and fucose-branched chain sulfation, as well as the number of fucose-branched chains and attachment sites. CD BioGlyco provides FCS production services for various species such as
- Apostichopus japonicus
- Cucumaria japonica
- Stichopus chloronotus
- Holothuria scabra
- Acaudina molpadioidea
- Holothuria fuscogilva
FCS extraction and degradation
FCS obtained based on different extraction methods have different structures and activities. our professional and well-trained team provides various FCS extraction and degradation services such as Enzymatic Extraction, alkaline extraction, Assisted Extraction, complex extraction, catalytic oxidation degradation, and nitrite degradation.
FCS isolation and purification
Our lab uses professional Chromatography Purification techniques for FCS purification. Our commonly used chromatography includes hydrophilic interaction chromatography (HILIC), strong anion-exchange chromatography (SAX), porous graphitic carbon chromatography (PGC), and ion pair reversed phase chromatography (IPRP).
FCS structural characterization and conformational analysis
FCS characterization: Our researchers provide advanced FCS characterization services by nuclear magnetic resonance (NMR) and tandem mass spectrometry (MS/MS or MSn). our lab has professional FCS multi-step derivatization strategies for structure identification. We provide FCS N-acetylation, carboxyamidation, permethylation, and silanization desulfurization modification services.
The fine structure of CS depends on the species and significantly influences the type and level of biological activity. We provide high-quality Antioxidant, anti-hyperlipidemia, Antiviral, Anticoagulation, anti-Parkinson's, Antitumor, and Antiinflammatory activity analysis services.
Publication
Technology: Polyacrylamide gel electrophoresis (PAGE), NMR spectroscopy, Gas-liquid chromatography(GLC)
Journal: Marine Drugs
Published: 2018
IF: 4.9
Results: The present study describes an FCS obtained from the sea cucumber Cucumaria djakonovi collected from the Avachinsky Gulf by enzymatic extraction, salt precipitation, and anion-exchange chromatography. The researchers characterized the structure of the FCS by NMR, GLC, and FTIR. The results showed that it contains the chondroitin core [→3)-β-D-GalNAc-(1→4)-β-D-GlcA-(1→]n. Moreover, analysis of the anti-inflammatory activity of sea cucumber-derived FCS and fish-derived CS demonstrates the enhanced biological activity of molecules containing sulfate-focused branches.
Fig.1 NMR spectra of FCS. (Ustyuzhanina, et al., 2018)
Applications
- FCS is a potentially novel selective inhibitor of the endogenous coagulation pathway.
- FCS effectively alleviates abnormal liver histology and regulates abnormal mRNA and lipogenesis-related hormones and inflammatory cytokines.
- FCS can be used in the development of antitumor drugs, and lipid-lowering drugs.
- GCS plays an important role in the regulation of key carbohydrate metabolism-related enzyme activities and induction of JNK and IKKβ/NFκB pathways.
Advantages of Us
- We offer FCS with good anticoagulant properties and reduced risk of pathogen contamination.
- The nitrite degradation services we offer without loss of fucosylated backbone or desulphurization, closely reflect the structure of natural FCS polysaccharides.
- Our lab offers a multi-step derivatization strategy that is ideally suited for accurate sulphation site analysis of isomers.
Frequently Asked Questions
What is the mechanism of anticoagulant action of FCS?
The mechanism of action of FCS anticoagulation is divided into antithrombin (AT) and heparin cofactor II (HCII)-independent anticoagulant factor X enzyme (FXase) activity, AT-dependent anticoagulant factor IIa (FIIa) or anticoagulant factor Xa (FXa) activity, and HCII-dependent anti-FIIa activity.
What are the FCS sulfate substitution sites?
The sulfate substitution of the GalNAc residue in the main chain of FCS occurs at the O-4, O-6, or O-4/6 positions. Sulfated fucose-branched chains can be linked to the O-4 or O-6 position of the GalNAc residue or the O-3 position of GlcA in the main chain of the FCS. The sulfate substitution in the fucose-branched chain occurs at the O-3, O-4, O-2/4, or O-3/4 positions.
Based on many years of production experience, CD BioGlyco provides precise and high-purity FCS production strategies to clients. We are committed to being your first choice for FCS production. Don't hesitate to contact us whenever you need assistance.
Reference
- Ustyuzhanina, N.E.; et al. Structure and anti-inflammatory activity of a new unusual fucosylated chondroitin sulfate from Cucumaria djakonovi. Marine Drugs. 2018, 16(10): 389.
