Full-scale Promotion of Chitooligosaccharide (CHOS) Production Relying on Skillful Processes
CD BioGlyco has high-quality Marine Biomolecule Production systems to meet the needs of different clients for Marine Carbohydrate Production, Characterization, and Activity Measurement. CHOS is a degradation product of Chitin or Chitosan extracted from Marine Animals. It is a positively charged oligosaccharide. Importantly, the deacetylation degree (DD), molecular weight (MW), and degree of polymerization (DP) of CHOS are closely related to its physical and biological activity. We offer high-yield and high-deacetylation CHOS production services.
CHOS preparation
- Chemical degradation: CD BioGlyco provides professional CHOS preparation via hydrogen peroxide oxidation or acid hydrolysis. Our commonly used acid hydrolysis includes hydrochloric acid, acids and electrolytes, nitrite, phosphoric acid, and hydrofluoric acid.
- Physical Degradation: CD BioGlyco provides reliable CHOS preparation service using microwave treatment, ultraviolet (UV) radiation, ultrasonic treatment, etc.
- Enzymatic Degradation: Depending on the needs of our clients, we offer a range of specific and non-specific enzymes for CHOS preparation. The enzymes we offer include chitinase, chitosanase, dextranase, lysozyme, protease, lipase, amylase, and so on.
- Electrochemical degradation: CD BioGlyco provides high-efficiency CHOS preparation using a Ti/TiO2-RuO2 electrode or Ti/Sb-SnO2 electrode.
CHOS purification
- CD BioGlyco provides reliable CHOS separation and purification services by Chromatography or Membrane Filtration Purification. Moreover, our lab provides isolation and preparation services for CHOS at a single DP or a specific molecular weight distribution.
CHOS characterization
- CD BioGlyco provides CHOS quantification services using ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
- CD BioGlyco provides CHOS polymerization, sequence, and structure analysis. Commonly used analytical tools in our lab include thin-layer chromatography (TIC), high-performance liquid chromatography (HPLC), electrospray ionization mass spectrometry (ESI-MS), Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS), ultraviolet/visible (UV-Vis), infrared radiation (IR), nuclear magnetic resonance (NMR) spectroscopy and Fourier transform infrared (FTIR) spectroscopy.
CHOS biological function analysis
Publication
Technology: FTIR spectroscopy, NMR spectroscopy
Journal: Foods
Published: 2023
IF: 5.2
Results: In this work, the researchers prepared CHOS by acid hydrolysis, oxidative hydrolysis, microwave, radiation, high-intensity ultrasound, and enzymatic hydrolysis. Moreover, the researchers also prepared CHOS derivatives with different modifications. It was found that the bioactivities such as anti-inflammatory, anticancer, antidiabetic, and other bioactivities of the modified CHOS derivatives were significantly enhanced.
Fig.1 Schematic diagram of CHOS production by hydrolysis. (Mittal, et al., 2023)
Applications
- CHOS can be used for antioxidant, antibacterial, and antifungal drug design and vector development for gene therapy applications.
- CHOS regulates glucose lipid metabolism by inhibiting the SMYD3 pathway and modulating intestinal flora.
- CHOS has immunomodulatory effects used to regulate blood glucose levels in diabetic patients.
- CHOS prevents colorectal cancer by reversing bacterial and fungal imbalances.
Advantages of Us
- The electrochemical degradation we offer without the addition of chemical or biochemical reaction reagents. It facilitates the separation and purification of the products, and the reaction is easy to control.
- The high-resolution characterization we provide is also applicable to CHOS mixtures.
- Our experienced researchers offer single degradation or combined degradation services depending on the client's needs.
Frequently Asked Questions
What is the chemical structure of CHOS?
CHOS is composed of homo- or hetero-oligomers of GlcNAc and D-glucosamine (GlcN), with a DP of 2~20.
Where does CHOS come from?
CHOS is a degradation product of chitin and chitosan. Chitin and chitosan are mostly derived from crustaceans such as shrimp crab or squid.
CD BioGlyco has many years of experience in marine polysaccharide production. Whatever the client's needs are for CHOS degradation, isolation, purification, characterization, or activity analysis, please feel free to contact us.
Reference
- Mittal, A.; et al. Chitooligosaccharide and its derivatives: potential candidates as food additives and bioactive components. Foods. 2023, 12(20): 3854.
