Relying on Advanced Production Technology to Provide You with Highly Active Heparin
Marine Animal Polysaccharide is a very rich class of Marine Biomolecule that has greatly contributed to the development of marine drugs. Heparin is a glycosaminoglycan, mainly composed of iduronic acid (IdoA), d-glucuronic acid (GlcA), α-d-glucosamine (GlcN) and its derivatives (acetylated, sulfonated). Due to its superior anticoagulant activity is widely developed into clinical drugs. Our professional Marine Carbohydrate Production team provides one-stop heparin production services such as extraction, isolation, purification, structural characterization, and activity testing according to client requirements. CD BioGlyco provides heparin extraction services from various sources including but not limited to:
- Mollusks heparin production
- Annelid heparin production
- Tunicate heparin production
- Crustacean heparin production
- Echinoderm heparin production
- Urochordate heparin production
Marine biological origin heparin extraction
CD BioGlyco extracts heparin using alkaline brine extraction, Enzymatic Extraction, and Ultrasonic-assisted Extraction without destroying as much as possible the physicochemical properties, structural features, and biological activities.
- Alkaline brine extraction: Our researchers have extensive operational experience in alkaline brine extraction. The alkali solution not only extracts heparin from the heparin-protein complex but also removes the protein by taking advantage of its reduced solubility in highly concentrated salt solutions and precipitating it.
- Enzymatic extraction: Our researchers offer a variety of degradative enzymes for heparin extraction depending on the source. We commonly use broad-spectrum proteases such as papain, trypsin, and chytridium neutralis. Moreover, our lab also offers complex enzyme extraction services.
- Ultrasonic-assisted extraction: We provide reliable ultrasonic extraction services with the help of skilled staff. We offer services for the optimization of influencing factors such as time, concentration, power, etc.
Marine biological origin heparin analysis
- We offer molecular weight and purity testing services for heparin by high-performance liquid gel chromatography. Our lab analyses its triple-stranded helical structure using Congo red assay.
- We offer heparin characterization and structural thermal stability analysis by infrared (IR) spectroscopy, nuclear magnetic resonance (NMR), and simultaneous thermal analyzer.
- Our researchers provide accurate monosaccharide and disaccharide composition analysis services.
- The biological activity of heparin is related to its structure, and we mainly provide the following activity analysis services as Anti-blood Clotting Activity Screening, Antitumor Activity Screening, and Antiinflammatory Activity Screening.
Publication
Technology: Alcian blue assay, Cellulose acetate electrophoresis, Infrared spectroscopy analysis, High-performance gel permeation chromatography (HPGPC), Fourier-transform infrared (FTIR) spectral analysis, NMR spectroscopy analysis,
Journal: Marine Drugs
Published: 2019
IF: 4.9
Results: In this work, researchers isolated natural heparin from Coelomactra antiquata by enzymatic digestion, ion exchange, and ethanol precipitation. The researchers not only characterized the structure of heparin by NMR, IR spectroscopy, and HPLC but also tested in vitro and in vivo fibrinolytic activity. The results showed that the isolated heparin had a molecular weight of 30.99 kDa and was mainly composed of →4)-α-IdoA2S-(1→4)-α-GlcNS3S6S (or GlcNS6S)-(1→4)-β-GlcA-(1→4)-α-GlcNS6S (or GlcNAC)-(1→. Heparin from Coelomactra antiquata has significantly higher molecular weight and fibrinolytic activity than terrestrial mammals. This provides important information for clinical and antithrombotic drug industry development.
Fig.1 Purification, HPGPC, electrophoresis, and IR spectrum of the clam heparinoid (G15). (Du, et al., 2019)
Applications of Heparin
- Heparin can be used to discover and develop specific and more effective antithrombotic drugs.
- Heparin can be used to develop a variety of marine health products and medicines, etc., realizing the in-depth development and utilization of marine biological resources.
- Heparin can be used as a substrate for heparin-degrading enzymes.
Advantages of Us
- CD BioGlyco not only provides the service of producing heparin from different sources but also provides the service of comparing the production rate, structure, molecular weight, and activity of heparin from different sources.
- The ultrasonic extraction method we offer has the advantages of no heating, high extraction rate, short extraction time, low impact on biological activity, and low solvent usage.
- Our professional production team offers small batch or commercial heparin production services according to client requirements.
Frequently Asked Questions
What are the advantages of extracting heparin from marine animals?
Heparin of marine biological origin, as an alternative raw material to conventional heparin, is characterized by low side effects and good compatibility with human cells. Marine biogenic heparin has a similar pharmacological activity to conventional heparin, but the anticoagulant effect is relatively moderate, which effectively reduces the bleeding side effects of its use.
What is the anticoagulant mechanism of heparin?
Heparin has a powerful anticoagulant effect both in vivo and in vitro. It carries a large negative charge and inactivates several coagulation factors. The anticoagulant effect is dependent on antithrombin III (AT III). It combines with thrombin through an arginine-serine peptide bond to form the At III thrombin complex and inactivate the enzyme, a reaction that is accelerated a thousandfold by heparin.
Relying on qualified production systems, CD BioGlyco provides one-stop solutions for heparin production to our clients all over the world. Whether you need low-molecular-weight heparin, medium-molecular-weight heparin, or high-molecular-weight heparin, please feel free to contact us.
Reference
- Du, Z.X.; et al. Isolation and characterization of a heparin-like compound with potent anticoagulant and fibrinolytic activity from the clam Coelomactra antiquata. Marine Drugs. 2019, 18(1): 6.
