Your Trusted Heparan Sulfate (HS) Production Partner
CD BioGlyco has professional Marine Carbohydrate Production experts to provide high-purity Marine Animal Polysaccharide Production service. Glycosaminoglycans (GAGs) are a group of Marine Biomolecules with specific biological activities. HS is a member of the GAG family of linear anionic polysaccharides. We not only provide HS extraction but also characterization services to further expand HS applications such as HS-based Drug-Delivery Vehicle Development. We provide HS isolation and purification from marine animals including but not limited to:
- Artemia franciscana HS production
- Amussium pleuronectus HS production
- Nodipecten nodosus HS production
- Litopenaeus vannamei HS production
- Portunus pelagicus HS production
- Perna canaliculus HS production
HS extraction
The structure and properties of HS from different species sources are different and therefore require different extraction methods. Our professional researchers provide GAG mixture extraction services by Organic Solvent Extraction, Enzymatic Extraction, and Assisted Extraction. Our staff provides you with the most suitable extraction solution based on preliminary experiments and reliable experience.
HS isolation
Our lab provides high-purity HS isolation services using gel filtration ion exchange chromatography, diethylaminoethyl (DEAE)-cellulose column Chromatography, and ethanol Precipitation Purification. We use (ultraviolet) UV detection for rapid identification of HS-containing fractions and collect them for desalination by dialysis using distilled water.
HS characterization
We provide high-level HS characterization services by disaccharide profile, elemental analysis, agarose and gradient polyacrylamide gel electrophoresis, and nuclear magnetic resonance (NMR) analysis. Our lab offers a wide range of services such as sulfation site analysis, disaccharide composition, purity determination, etc.
Publication
Technology: Agarose gel electrophoresis, Attenuated Fourier-transform infrared spectroscopy (FTIR) spectral Analysis, NMR, Constituent disaccharide analysis, Förster resonance energy transfer, Circular dichroism spectroscopy, Differential scanning fluorimetry, Activated partial thromboplastin time (aPTT), Prothrombin time (PT), Principal component analysis (PCA)
Journal: Marine Drugs
Published: 2019
IF: 4.9
Results: In this study, the researchers used ion exchange chromatography and sodium chloride gradient separation to obtain a HS-containing GAG extract from Portunus pelagicus. Fraction 5 (F5) was identified as HS by electrophoresis, FTIR, and NMR. The researchers tested the activity of F5 and found it to have potent beta-site amyloid precursor protein cleaving enzyme 1 (BACE1) inhibitory activity with highly attenuated anticoagulant activity. The mechanism of inhibition of the F5 is complex and different from that of heparin. This provides a potential starting point for marine GAG drug development.
Fig.1 FTIR spectra and PCA of P. pelagicus F5. (Mycroft-West, et al., 2019)
Applications
- HS plays an important role in the development of anti-inflammatory therapeutics.
- HS is a potential target for anticoagulant drug development.
- HS can be used to research the conformational relationship of marine polysaccharides.
- HS plays a key role in viral infections and can be used to develop antiviral drugs.
Advantages of Us
- CD BioGlyco has accumulated many years of experience in HS extraction, isolation, purification, and characterization.
- The HS we provide with high-yield and high activity.
- Our researchers pay extra attention to isolation and purification to ensure the delivery of high-purity HS.
- Our reliable team is skilled in the key processes of HS production to help our clients produce HS with different characteristics.
Frequently Asked Questions
What are the structural compositional peculiarities of HS?
HS is dominated by disaccharide units consisting of β-D-glucuronic acid and 2-N-acetylamino-2-deoxyα-D-glucose (including the O-sulfate substitution of C6) linked by (1→4). HS also contains β-D-glucuronic acid or aldose linked to 2-N-sulfo-2-deoxyα-D-glucose (including the O-sulfate substitution of C6).
What is the principle of polysaccharide separation on the DEAE-cellulose column?
DEAE-cellulose has an open backbone, and polysaccharides enter the carrier freely and diffuse rapidly. Its separation mechanism is not a single ion exchange, but adsorption and desorption. The components to be separated in the solution are adsorbed on the ion exchanger by the Coulomb force and charge difference, and the suitable eluent is selected to elute the adsorbent on the ion exchanger to achieve the purpose of separation. Using different ionic strength solutions to elute step by step, to get different components of polysaccharide and achieve the separation effect.
CD BioGlyco provides efficient and reliable carbohydrate production services to accelerate our clients' scientific research by reliable staff. We have a knowledgeable operational team to help clients plan and complete projects. Our efficient service has been praised by our clients all over the world. No matter what problems you encounter in HS production, please feel free to contact us.
Reference
- Mycroft-West, C.J.; et al. A glycosaminoglycan extract from Portunus pelagicus inhibits BACE1, the β secretase implicated in Alzheimer's disease. Marine Drugs. 2019, 17(5): 293.
