Satisfactory Hyaluronan Production Program at CD BioGlyco
CD BioGlyco has accumulated many years of experience in Marine Biomolecule Production to provide our clients with the most satisfactory services in the production, characterization, and activity testing of Marine Carbohydrate. Marine Animals are an important source of glycosaminoglycan production. Hyaluronan is an important class of glycosaminoglycans, which consists of the disaccharide units D-glucuronic acid and N-acetylglucosamine, a class of advanced polysaccharides with disaccharides as monomers. Different molecular weights of hyaluronan are used in very different applications. Therefore, our highly trained team offers low molecular weight hyaluronic acid (LMW-HA) production, medium molecular weight hyaluronic acid (MMW-HA) production, and high molecular weight hyaluronic acid (HMW-HA) production services.
Hyaluronan extraction and purification
- Our experienced staff provides a range of services such as animal tissue pre-treatment, degreasing, precipitation, deproteinization, and extraction. Our lab provides a full range of hyaluronan extraction services including Organic Solvent Extraction, Enzymatic Extraction, or Assisted Extraction.
- Relying on a proven animal polysaccharide production system, we provide high-purity hyaluronan purification services through Chromatography Purification (ion exchange chromatography and high-performance liquid chromatography (HPLC)), Precipitation Purification (isoelectric point precipitation, alcohol precipitation, and quaternary ammonium precipitation), Ultracentrifugation Purification, Membrane Filtration Purification, and Preparative Gel Electrophoresis Purification. Separation of target polysaccharides from impurities and other polysaccharides using differences in molecular weight size, and charge density.
Hyaluronan analysis
- Our lab provides professional hyaluronan purity determination, alduronic acid content analysis, and yield determination services. Moreover, we use mass spectrometry (MS), nuclear magnetic resonance (NMR), and Fourier transform infrared (FTIR) spectroscopy to analyze hyaluronan structure.
- Hyaluronan has various biofunctional properties such as good permeability, antioxidant, anti-inflammatory, immunosuppressive, and anti-angiogenic properties. We provide high-quality bioactivity evaluation services such as Antioxidant Activity, Anti-blood Clotting Activity, and Antiinflammatory Activity.
Publication
Technology: Organic solvent extraction, Enzymatic extraction, Precipitation purification, Ultrafiltration-diafiltration technologies (UF-DF)
Journal: Marine Drugs
Published: 2013
IF: 4.9
Results: This article describes a strategy to extract hyaluronan from the vitreous humor (VH) of fish eyes. Researchers combined enzymatic and organic solvent precipitation methods to extract mixed polysaccharides from frozen eyeballs (tuna, shark, and swordfish). Hyaluronan of 99.99% purity (molecular weight 2000 kDa) was obtained after dissolution, neutralization, UF-DF separation, purification, and concentration.
Fig.1 Schematic diagram of hyaluronan extraction and purification from fish eyeball. (Vázquez, et al., 2013)
Applications
- Hyaluronan can be used as a breast or prostate cancer marker and to monitor the course of the disease.
- Hyaluronan is used as a useful additive in nutritional supplements and cosmetics.
- Hyaluronan can be used to develop anti-cancer drug delivery systems.
- Hyaluronan can be used to synthesize bioscaffolds with healing properties.
- Hyaluronan regulates collagen synthesis, inhibits inflammatory responses, and aids in wound healing.
Advantages of Us
- We offer assisted extraction methods that reduce processing time, minimize solvent use, and increase hyaluronic acid yields.
- Our skilled operators provide LMW-HA with important antioxidant and anti-glycosylation activities.
- Our researchers have proven experience in the isolation and purification of hyaluronan from crude glycosaminoglycans and reliable isolation conditions.
- Our staff has accumulated many years of operational experience in the extraction and purification of hyaluronan.
Frequently Asked Questions
What are the sources and specialties of marine animal hyaluronan?
The main sources of hyaluronan from marine animals include salmon, trout, shark, swordfish, zebrafish, molluscan bivalves, stingrays, and shellfish (cartilage, head, liver, eyes, fins, and skin). It is structurally similar to hyaluronan extracted from mammals such as roosters or cows. A significant difference is that the molecular weight of hyaluronan from marine sources is smaller than that of hyaluronan from mammalian sources.
What are the properties of the different molecular weights of hyaluronan?
- The molecular weight of LMW-HA ranges from 10,000 to one million. It has a small molecular size and low viscosity, and easily penetrates skin layers and tissue cells. LMW-HA has anti-tumor, wound healing, angiogenesis, and immunomodulatory effects.
- The molecular weight of MMW-HA is between 1 and 10 million molecules. MMW-HA has a high viscosity and water retention capacity and is capable of forming gelatinous substances.
- The molecular weight of HMW-HA is more than 10 million. HMW-HA has a large molecular size and high viscosity, which can form a protective barrier on the skin surface. It has better viscoelasticity, moisturization, inhibition of inflammatory reactions, lubrication, and slow release of drugs.
CD BioGlyco has team members with different professional backgrounds working together to help our clients with hyaluronan production projects. Our staff responds quickly to your every need. For any further information, do not hesitate to contact us.
Reference
- Vázquez, J.A.; et al. Chondroitin sulfate, hyaluronic acid and chitin/chitosan production using marine waste sources: Characteristics, applications and eco-friendly processes: A review. Marine Drugs. 2013, 11(3): 747-774.
