Agelasphin Production: Unleashing Nature's Potent Antitumor Agents
Through our advanced Marine Biomolecule Production Platform, CD BioGlyco offers a specialized production service for agelasphin, a unique class of glycosphingolipid derived from marine sources. They are notably characterized by the presence of a galactosyl (galactose) unit, diverging from the more ubiquitous glucosyl (glucose) moiety found in related compounds. This structural divergence endows agelasphins with unique and intriguing biological properties. We have the ability to produce many types of agelasphins, including agelasphins 7a, agelasphins 7b, agelasphins 9a, agelasphins 9b, agelasphins 10, agelasphins 11, and agelasphins 13.
Our services cover extraction and synthesis methods for creating various agelasphins to meet the diverse requirements for research and practical use.
Production by Extraction Method
Our extraction approach involves isolating agelasphins directly from marine sources by advanced techniques to ensure high purity and yield.
- Extraction: The sponge Agelas mauritianus is freeze-dried, and its compounds are extracted using a 1:1 mixture of chloroform and methanol. The extract will be separated into different layers by partitioning with ethyl acetate and water.
- Purification: We use the aqueous methanol to wash the ethyl acetate layer, which contains the lipophilic compounds, to remove more polar substances. The remaining lipophilic fraction will be subjected to multiple rounds of chromatography on silica gel. This results in a fraction that is primarily composed of homologous glycosphingolipids. Then we use reversed-phase high-performance liquid chromatography (RP-HPLC) to separate every type of agelasphin.
Production by Synthetic Method
Our research team will design appropriate synthetic routes to offer personalized agelasphin synthesis based on the client's unique requirements. Whether clients need specific structural configurations or unique Chemical Modifications, our team will meet clients' requirements.
After extraction or synthesis, we conduct a thorough analysis of the resulting substance to ensure its quality and accuracy. This includes a range of analytical techniques to verify the chemical structure, purity, and overall composition of the product. We usually use techniques like nuclear magnetic resonance (NMR), mass spectrometry (MS), HPLC, and infrared spectroscopy (IR) in our analysis.
Publication Data
DOI: 10.3390/molecules181215662
Journal: Molecules
Published: 2012
IF: 4.2
Results: In this article, authors delved into the realm of natural killer T (NKT) cells and their capacity to recognize glycolipid antigens, with a particular emphasis on recent advancements in research concerning glycolipids sourced from marine sponges. The NKT cells were explained to play a critical role in connecting innate and adaptive immune responses by interacting with glycolipids displayed by the CD1d molecule. The authors mentioned that a new type of glycolipid called "agelasphin" was discovered from marine sources. These saccharides are linked to a ceramide backbone. The derivative of agelasphin, KRN7000, was developed from a structure-activity study. Then it was identified as a key antigen for CD1d-restricted iNKT cells, making it important for studying iNKT cell activation. Furthermore, the article elaborated on structure-activity relationship (SAR) investigations that pinpoint critical structural elements necessary for glycolipid efficacy and examine the involvement of NKT cells in a spectrum of diseases.
Applications
- Agelasphin can be used as an effective antitumor agent due to its ability to selectively target and inhibit cancer cell growth.
- Agelasphin can be applied to synthesize analogs or derivatives that might offer enhanced biological activity or reduced toxicity.
- Agelasphin is useful for its anti-inflammatory properties, providing insights into its influence on inflammatory pathways.
Advantages
- We utilize a sophisticated production platform to ensure the high-quality production of diverse agelasphins, with each type characterized by distinct biological properties.
- We offer personalized synthesis routes designed to meet specific client requirements, including unique structural configurations and chemical modifications of agelasphins.
- We possess flexibility in production scales and techniques to cater to both research and practical applications, thereby providing tailored solutions to meet a variety of requirements.
Frequently Asked Questions
What are the biological activities of agelasphins?
Agelasphins exhibit a spectrum of biological activities, including antitumor, anti-inflammatory, and immunomodulatory effects.
How does agelasphin exert its anti-tumor effects at the molecular level?
In the intricate dance of molecules, the agelasphins' remarkable immune-stimulating properties manifest. These compounds, powerful ligands for the MHC class I-like CD1d protein adorning the surface of antigen-presenting cells (APCs), ignite a robust immune response. This intense interaction triggers a cascade of events, primarily activating the iNKT cells, which burst forth with copious cytokines. These cytokines, in turn, mobilize other antitumor effector cells, orchestrating a potent immunological symphony against tumor cells within the organism.
CD BioGlyco's production encompasses both extraction and synthesis techniques for addressing a spectrum of research and application demands. The extraction method involves the direct isolation of agelasphins from marine sources, employing cutting-edge technologies to achieve exceptional purity and yield. Alternatively, our synthesis approach facilitates laboratory-based production of agelasphins, granting precise control over their structural and compositional attributes. Both methodologies benefit from our advanced facilities and a highly skilled team. Please feel free to contact us to learn more about our other Marine Glycolipid Production Services.
Reference
- Anderson, B.L.; et al. Stimulation of natural killer T cells by glycolipids. Molecules. 2012, 18(12): 15662-15688.
