Excellence in Terpioside Production
Terpioside, derived from the marine sponge Terpios sp., is a unique Glycosphingolipid that contains a residue structure in the form of furanose in its pentasaccharide chain. It is also a potent inhibitor against lipopolysaccharide-induced NO release. CD BioGlyco has accumulated a wealth of experience and received wide acclaim in the field of Marine Glycolipid Production. Here, we provide our clients with trustworthy terpioside production service.
Extraction and Isolation of Terpioside
- We break the marine sponge Terpios sp. and add the appropriate amount of chloroform and methanol mixture to it to extract terpioside.
- All mixtures are centrifuged and the supernatant is collected.
- The supernatant is extracted using BuOH and H2O and the organic fractions are collected.
- The organic fractions are purified sequentially by reversed-phase chromatography and forward chromatography to obtain the crude product of terpioside.
The crude product of terpioside is eluted with trichloromethane on a silica gel column, and the eluate is collected and concentrated. The concentrated terpioside-containing fraction is then processed using high-performance liquid chromatography (HPLC) to obtain terpioside as a pure product.
We use 2D nuclear magnetic resonance (NMR), and microscale degradation to analyze the structure of terpioside, spectroscopy to analyze the presence of optical isomers of terpioside, and also gas chromatography-mass spectrometry (GC-MS) and HPLC to test the purity of terpioside.
Applications
- The structure of terpioside may interfere with the signaling pathways of tumor cells, thereby inhibiting their growth and spread, and thus it could be used in the development of novel anticancer drugs.
- Terpioside shows some immunomodulatory effects, which can affect the function and signaling of immune cells, and can be used in the research of immune-related diseases.
- Due to its unique and complex chemical structure, terpioside can be used as a lead compound for the synthesis of novel drugs. Scientists can use it as a basis for structural modification to develop new drugs with higher potency and lower side effects.
Advantages
- Our Marine Biomolecule Production team with many years of hands-on experience continuously optimizes the terpioside production process to provide high-quality terpioside products.
- We have experts in biotechnology, chemical engineering, and quality control to provide custom terpioside production solutions for our clients.
- We strictly control the quality of our products to ensure the consistency and purity of each batch of terpioside products.
Frequently Asked Questions
What are the potential activities of terpioside?
Terpioside counteracts lipopolysaccharide-induced NO release and is a potent inhibitor. In addition, it has antimicrobial, anticancer, anti-inflammatory, immunomodulatory, antiviral, and antioxidant activities.
What are the advantages of terpioside over other glycolipids?
Terpioside may have multifunctional properties such as anticancer, antibacterial and antiviral activity, and immunomodulatory properties. In addition, certain research findings suggest that terpioside exhibits a high degree of selectivity and safety with low toxicity to normal cells over a range of effective concentrations.
With advanced technology and a top-notch team, CD BioGlyco provides one-stop solutions for terpioside production, characterization, and Bioactivity Analysis. Our production process is precise and stable to ensure high-quality terpioside products and provide strong guarantees for our clients. We welcome every client to contact us at any time and let's work together to promote the development of the marine biomolecule industry.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
