Your Trusted Partner in Marine Gangliosides HLG Production
Gangliosides is a glycosphingolipid that is highly abundant in the nervous system and plays an important role in the regulation of membrane proteins and ion channels, cell signaling, and intercellular communication. Gangliosides HLG from sea cucumber has a unique structure with favorable physiological activities. At CD BioGlyco, we have a team of experts specializing in Marine Glycosphingolipid research, and we have abundant resources in Marine Glycolipid Production to provide one-stop marine gangliosides HLG production to our clients.
Direct Extraction and Purification of Marine Gangliosides HLG
- The sea cucumber is chopped and extracted several times with a mixture of chloroform and methanol in a certain proportion. After combining the extracts, vacuum concentration is performed and appropriate amounts of distilled water, hexane, and acetone are added to them sequentially. Then, the organic phase is collected and the solvent is removed to obtain a crude product of gangliosides HLG.
- The purification of gangliosides HLG is carried out using continuous column chromatography, in which silica gel is used as the stationary phase, and a mixture of methanol, chloroform, and water in different ratios is used as the mobile phase. Meanwhile, the purification results are detected using thin-layer chromatography (TLC).
Total Synthesis of Marine Gangliosides HLG
- Marine gangliosides HLG consists of a glycan structure and a ceramide structure. We assemble HLG by a stereoselective reaction process with the glycosyl compound using an amide containing lactam glucoside as the glycosyl acceptor, i.e., the ceramide portion. This method allows us to obtain HLG in high yields.
- For the purification of HLG, we use column chromatography. In this, a mixture of water, methanol, and chloroform is used as the eluent, and silica gel is used as the stationary phase.
Characterization of Marine Gangliosides HLG
- We use nuclear magnetic resonance (NMR) techniques, infrared (IR), and mass spectrometry (MS) to characterize the structure of HLG.
- The molecular weight of HLG is determined by high-resolution mass spectrometry (HRMS).
- The molecular formula of HLG is determined by elemental analysis combined with HRMS.
- An optical rotator is used to test the rotation of HLG.
Applications
- Gangliosides HLG has neuroprotective and neurorestorative potential and can be used in research on neurodegenerative diseases.
- Gangliosides HLG can modulate the immune system response and can be used in research on certain autoimmune and inflammatory diseases.
- Gangliosides HLG is involved in cellular signaling and cell-cell interactions, so it can be used to develop carriers for drug delivery systems to help deliver drugs accurately to target cells or tissues.
Advantages
- Our Marine Biomolecule research team has mastered advanced extraction and purification technologies to help our clients produce high-purity HLG.
- Our team covers experts in multiple fields including chemistry, biology, and oceanography, providing guidance on the production and application of HLG, as well as ensuring the high efficiency and stability of the HLG production process.
- We provide full support to our clients, including but not limited to the interpretation of data related to HLG products, the use of HLG products, and after-sales service, to ensure that our clients can maximize the use of our products.
Frequently Asked Questions
What are the physiological activities of marine gangliosides HLG?
Neuroprotective effects: Marine gangliosides HLG helps to promote the growth and repair of nerve cells, and can increase the survival rate of nerve cells.
Immunomodulatory effects: Marine gangliosides HLG exhibit various functions in immunomodulation, such as inhibiting or promoting the proliferation and activity of immune cells.
Antioxidant properties: Marine gangliosides HLG protects cells from oxidative stress damage, thus slowing down cellular aging.
What are the advantages of direct extraction and chemical total synthesis in the production of gangliosides HLG?
- Direct Extraction
- Lower production cost: The process of direct extraction is relatively simple and less expensive than complex chemical synthesis.
- Reduced by-products: The direct extraction method may produce fewer by-products and impurities, which is relatively more environmentally friendly and less toxic.
- Chemical Total Synthesis
- High purity: The chemical synthesis process can strictly control the reaction conditions to produce highly pure gangliosides HLG with fewer impurities and by-products.
- Scale production: Due to the highly controllable production process, chemically synthesized gangliosides HLG products have excellent batch consistency and are suitable for large-scale industrial production.
- Highly customizable: It can be customized by chemical modification to generate derivatives with similar structures but different functions to meet specific research or application needs.
CD BioGlyco has superior technology and a dedicated team in the field of marine gangliosides HLG production. We specialize in innovative and efficient production methods to ensure that the quality and purity of our HLG products always meet the highest standards. Please feel free to contact us if you have any questions about this.
Reference
- Hayashi, T.; et al. Halogen-directed chemical sialylation: pseudo-stereodivergent access to marine ganglioside epitopes. Chem. Sci. 2020, 11: 6527-6531.
