Vesparioside Production: Excellence in Every Step
Vesparioside, derived from the marine sponge Spheciospongia vesparia, is a Marine Glycosphingolipid with a pentose residue structure. vesparioside has been categorized by scientists as an area of special interest due to its complex structure and immunomodulatory properties. At CD BioGlyco, we rely on state-of-the-art Marine Glycolipid Production methods to provide vesparioside production services to our clients and to help them understand and apply the functions of vesparioside.
Extraction and Isolation of Vesparioside
We use a mixture of chloroform and methanol as an extraction solvent to extract vesparioside from the sponge Spheciospongia vesparia. At the end of the extraction, chloroform and methanol are removed to obtain the crude product of vesparioside.
Purification of Vesparioside
- The crude extract of vesparioside is extracted using a mixture of water and BuOH and the organic phase is collected.
- The organic phase is concentrated in a vacuum and then purified sequentially using silica gel and silica columns, a step that effectively removes non-glycolipid impurities.
- To keep the structure of vesparioside from being destroyed in the subsequent purification step, we perform acetylation of the glycolipid mixture. Afterward, high-performance liquid chromatography (HPLC) is used to remove other glycolipid compounds, which in combination with the deacetylation operation results in pure vesparioside.
Characterization of Vesparioside
- Structure
Vesparioside has a complex and specific structure, so we analyze its structure using microscale chemical degradation combined with mass spectrometry (MS), nuclear magnetic resonance (NMR), and circular dichroic (CD) spectrum. That is, we perform acidic decomposition of vesparioside and use MS, NMR, and CD spectrum to analyze the structure of the product and the conformation of the sugar chain. Finally, the structural characterization of vesparioside is deduced from all the results.
- Purity
We utilize HPLC to test the purity of vesparioside to ensure that the vesparioside produced meets the purity requirements of our clients.
Publication Data
Technology: Solvent extraction and spectroscopy
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors show the structural features of the marine glycosphingolipid vesparioside, which was isolated from the marine sponge Spheciospongia vesparia after an organic solvent extraction method. After analysis using spectroscopic techniques, it was found to be a unique disaccharide glycosylated marine glycosphingolipid with fragments of furanose residues in its structure.
Fig.1 Structures of vesparioside. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Vesparioside possesses anti-tumor activity, inhibiting the growth and proliferation of many types of cancer cells, which allows it to be used in the development of novel anti-cancer drugs.
- Vesparioside shows immunomodulatory effects and can be used in research on the immune response of the organism.
- Vesparioside has a protective effect on nerve cells, which can be used in the research of neurodegenerative diseases.
Advantages
- We have an experienced Marine Biomolecule research team with cutting-edge chemical and biological technologies to understand and optimize the production and analytical processes of vesparioside.
- We have a strict quality control system that continuously monitors and verifies parameters during the production process to ensure the purity and activity of vesparioside.
- We provide custom vesparioside production solutions to produce vesparioside efficiently and reliably, meeting the specific needs of different industries and application scenarios.
Frequently Asked Questions
What is the structure of vesparioside?
Vesparioside is a natural glycosphingolipid containing pentose residues and consisting of a series of homologs with different lipid chain structures. It is rich in furanose structures, multiple 1,2-cis-glycosidic bonds, and has a 2,3-branched sugar structure at the non-reducing end.
How to customize marine glycosphingolipid products?
The general process of customizing marine glycosphingolipid products usually includes the following steps:
- Demand analysis and communication: Communicate with clients to gain an in-depth understanding of their specific needs.
- Program design and planning: Develop a detailed technical program, including product specifications and production processes.
- Production: Production of marine glycosphingolipid products, comprehensive testing of the product, and necessary adjustments to the design based on test results.
- Quality control and inspection: Final inspection of the finished product to ensure that the product meets the specifications and quality standards.
- Product delivery: Deliver the products to clients on time, and provide necessary product instructions and usage guidelines.
- After-sales service: Provide timely after-sales service, deal with the problems encountered by clients in the process of use, and ensure client satisfaction.
CD BioGlyco provides efficient and reliable vesparioside production services to clients in a variety of industries with industry-leading technology and an outstanding team. We are committed to providing custom solutions to meet a variety of research and production needs. We welcome clients to contact us at any time, and our professional team will be happy to provide you with advice and support.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
