Superior Marine Ganglioside CG-1 for Ultimate Innovation
Ganglioside CG-1 is a class of Marine Glycosphingolipid from the marine organism sea cucumber, which has good neuromodulatory activity. CD BioGlyco is a professional biology company that provides various Marine Biomolecule Production Services. With advanced Marine Glycolipid Production technology, we provide custom Ganglioside CG-1 production solutions to our clients to help them extract ganglioside CG-1 efficiently.
Extraction of Ganglioside CG-1
First, we cut the sea cucumber into pieces, and then the CG-1 is extracted several times using a specific ratio of CHCl3/MeOH. After the extraction, the extracts are combined and concentrated under a vacuum. After that, the organic phase is collected by multiple extractions with AcOEt/n-BuOH. The crude product of CG-1 is obtained after removing the solvent from the organic phase.
Purification of Ganglioside CG-1
The obtained crude CG-1 is purified several times by silica gel-gel column chromatography and the results are analyzed using thin-layer chromatography (TLC) (i.e., pure CG-1 has only one spot on the TLC plate). Notably, during the purification process, to ensure that the impurities could be completely removed, we use CHCl3/MeOH for its gradient elution.
Characterization of Ganglioside CG-1
- Structure
We use nuclear magnetic resonance (1H NMR and 13C NMR), infrared spectroscopy (IR), and mass spectrometry (MS) to characterize the structure of ganglioside CG-1 and analyze its functional groups.
- Melting Point
A melting point meter is used to test the melting point (m.p.) of ganglioside CG-1. The m.p. of ganglioside CG-1 should be between 136-139°C.
- Relative Molecular Weight
Fast-atom-bombardment MS (FAB-MS) is used to analyze the relative molecular weight of ganglioside CG-1.
- Purity
High-performance liquid chromatography (HPLC) is used to test the purity of ganglioside CG-1.
Publication Data
Technology: Solvent extraction method
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors describe the origin and bioactivity of ganglioside CG-1, which was isolated from sea cucumber using organic solvents and tested for bioactivity and found to have neurogenic activity against rat PC-12 cells. In addition, the authors describe a strategy for the synthesis of relevant CG-1 compounds lacking the sulfate moiety in natural products: coupling of 2-mercapto-glycosides with ganglioside analogs in the presence of NIS/TfOH, followed by stepwise deprotection, to obtain relevant CG-1 compounds.
Fig.1 The chemical structure of CG-1. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Marine ganglioside CG-1 has the potential to promote neuronal survival and axonal regeneration, so it can be used in research for the treatment of neurological injuries and degenerative diseases.
- Marine ganglioside CG-1 can be used to research pathological changes within the nervous system for early diagnosis and monitoring of disease course.
- Marine ganglioside CG-1 promotes structural and functional restoration of cell membranes and can be used in research on cell membrane disruptive diseases such as certain types of poisoning or trauma.
Advantages
- We have a marine glycolipid R&D team of biologists and chemists to provide cutting-edge scientific guidance and technical support to our clients to ensure that they effectively utilize marine ganglioside CG-1 products.
- We provide custom marine ganglioside CG-1 extraction, purification, and characterization solutions in one package to meet different application requirements.
- We have an advanced quality control system to ensure the high quality and consistency of each batch of marine ganglioside CG-1 products.
Frequently Asked Questions
Why choose to use marine-derived ganglioside?
Marine-derived ganglioside has a unique chemical structure and biological activity that differs from gangliosides from terrestrial sources, which may lead to novel and effective biomedical applications. Many marine-derived gangliosides have shown superior neuroprotective effects in research and can be used to prevent and treat neurodegenerative diseases such as Alzheimer's and Parkinson's diseases. In addition, the production of gangliosides from marine organisms may employ novel synthetic pathways, the development of which would not only help to obtain highly efficient bioactive but could also lead to new biotechnological and bioengineering approaches.
What are the biological activities of marine ganglioside CG-1?
Marine ganglioside CG-1 has a variety of biological activities, such as:
- Neuroprotection: Marine ganglioside CG-1 can promote neuronal growth, differentiation, and repair at the cellular level, and has a protective effect on nerve cells.
- Immunomodulation: Marine ganglioside CG-1 can regulate the function of the immune system and enhance the body's resistance to diseases.
- Anti-inflammatory effects: Marine ganglioside CG-1 has the function of reducing inflammatory reactions, and can be used to treat and alleviate inflammation-related diseases, such as arthritis and other chronic inflammatory conditions.
- Antioxidant effects: Marine ganglioside CG-1 exhibits significant antioxidant capacity to scavenge harmful free radicals from the body, thereby protecting cells from oxidative stress and damage.
CD BioGlyco is committed to providing the best quality marine ganglioside CG-1 products and the most professional service to our clients, and we also design the production program according to our client's needs. We value every client and maintain close communication with them, responding to needs and feedback on time to ensure that every batch of marine ganglioside CG-1 products meets clients' expectations. Please feel free to contact us to discuss more cooperation opportunities and technical details.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
