Excellence in Axinelloside Production
Axinelloside A is a highly sulfated glycosphingolipid from marine sponges that can be used to develop human telomerase inhibitors. CD BioGlyco has assembled a team dedicated to Marine Biomolecule research and has mastered advanced Marine Glycosphingolipid Production technology to provide a full range of axinelloside A extraction, purification, and characterization services to our clients.
Extraction of Axinelloside A
We use a mixture of methanol, ethanol, and acetone to extract axinelloside A from sponge fragments. After several extractions, we combine all the extracts and perform a second extraction using water and ether. After the extraction is completed, the organic phases are collected together and the solvents are removed under vacuum to give a crude product of axinelloside A.
Purification of Axinelloside A
We use silica gel column chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC) to purify axinelloside A. Throughout the purification process, we use mixed organic solvents as eluents, while using gradient elution method for elution. The purified axinelloside A is a colorless amorphous solid.
Characterization of Axinelloside A
- Molecular weight: We analyze the molecular weight of axinelloside A using nuclear magnetic resonance (NMR) combined with electrospray-ionization quadrupole ion trap mass spectrum (ESI-QIT-MS).
- Structural: The structure of axinelloside A is analyzed using 2D-NMR, infrared (IR), and MS.
- Sugar composition: To determine the absolute composition of sugars, we convert acid hydrolysates of axinelloside A to alditols, which are analyzed using gas chromatography (GC) and GC-MS. In addition, we determine the sugar conformation using Hara's method.
Publication Data
Technology: Solvent extraction, NMR and MS
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors describe the extraction, structural characterization methods, and biological activity of axinelloside A. Axinelloside A is a sulfated sphingolipid that can be extracted by solvent extraction from the marine sponge Axinella infundibula. NMR and MS can be used to fully characterize its structure. The structure of axinelloside A was found to be similar to the sulfated polysaccharides in the marine sponges Chondrilla nucula and Dysidea fragilis by both methods. Meanwhile, the results of the bioactivity assay indicated that axinelloside A strongly inhibits human telomerase with IC.
Fig.1 The chemical structure of axinelloside A. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Axinelloside A has a strong inhibitory effect on human telomerase, which makes it useful for the development of human telomerase inhibitors.
- Axinelloside A has some antibacterial and antiviral activity, which makes it useful in research on infectious diseases.
- Axinelloside A can be used to research cellular signal transduction and metabolic pathways, thus revealing new molecular mechanisms.
Advantages
- Our state-of-the-art, modern Marine Glycolipid Production technology ensures that our clients are supplied with high-purity axinelloside A products.
- We have a personalized service, which means that we provide custom axinelloside A production solutions and technical support according to our client's needs.
- We have a full-process quality control system to ensure the high standard and quality of axinelloside A products from development, production to use.
Frequently Asked Questions
What are the molecular structural features of axinelloside A?
Axinelloside A is a complex, highly sulfated oligosaccharide with multiple glycosyl structures such as d-arabinose, five d-galactose, and five l-fucose units.
What are the known biological activities of axinelloside A?
Axinelloside A is a rare natural product with a variety of biological activities such as immunomodulation, anti-tumor, antibacterial, and antiviral activities.
CD BioGlyco is committed to providing high-quality axinelloside A production services to global clients based on superior marine glycosphingolipid production technology, a professional team, and efficient service. Please feel free to contact us if you have any needs or questions in this area, our staff will answer your questions in time.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
