Amplify Your Science with Amphiceramide Excellence
At CD BioGlyco, we help clients all over the world to produce Marine Glycosphingolipids, that represent a diverse class of biomolecules, fundamentally characterized by their dual structural components: a sugar moiety and a ceramide base. The ceramide segment is composed of a sphingoid backbone coupled with an amide-linked fatty acyl chain. Generally, the amphipathic nature of glycosphingolipids underpins their broad spectrum of biological activities. In marine ecosystems, these compounds have been identified in a variety of organisms, including algae, sponges, echinoderms, and fungi. Therefore, we have developed an advanced platform to provide manufacturing services for various Marine Glycolipids. Amphiceramide described on this service page is a diosylceramide, a kind of glycolipid. We extract it from marine organisms to facilitate its production.
Acquisition of Raw Materials
Our researchers obtain specimens of Amphimedon compressa sponge with high quality as our starting material. We maintain strict control over the preservation of raw materials, that remain frozen until the extraction process, ensuring that their biological characteristics are maintained throughout the period of storage and subsequent analysis.
Extraction of Amphiceramide
Firstly, the sponge is homogenized to break it down into a uniform mixture. Then we will conduct a series of extractions using methanol, which is followed by chloroform. After completing these extraction steps, perform the partitioning process on the combined extracts, which will be separated into two distinct layers: one in water and the other in n-butanol.
Isolation of Amphiceramide
We concentrate the organic layer under reduced pressure and the organic layer will form a dark oil. Subsequently, we use a silica gel column to conduct the steps of initial purification through a chromatographic process, which results in a rude mixture.
Purification of Amphiceramide
The mixture is further purified on a SiO2 column to yield a glycolipid-rich fraction. Then we peracetylate the fraction and separate it by high-performance liquid chromatography (HPLC) on a SiO2 column using an n-hexane/ethyl acetate mixture. After this process, we obtain the amphiceramide with high purity.
Analysis of Amphiceramide
- Our research team determines the molecular weight by using a mass spectrometer for high-resolution data.
- Its optical rotations will be also measured through a polarimeter.
- Besides, we record 1H and 13C spectra, referencing chemical shifts to solvent signals and transforming the data for accurate coupling constant measurement. We also determine the homonuclear and through-space 1H connectivities using COSY, TOCSY, and ROESY experiments, optimizing HSQC and HMBC spectra for specific coupling constants. Through these detections, we analyze its structure accurately.
Publication Data
DOI: org/10.3390/md21100539
Journal: Marine Drugs
Published: 2018
IF: 4.9
Results: The text delves into the intricate chemistry and biological significance of glycolipids, particularly those sourced from marine environments. Glycolipids encompass a wide-ranging collection of natural compounds, where a glycosidic fragment is intricately linked to a lipid molecule. These substances are primarily divided into three principal categories: glycosphingolipids, glycoglycerolipids, and atypical glycolipids. In the review, the authors described the rich structural diversity and biological functions of these entities and highlighted their promising potential as targets for synthetic chemistry endeavors. They mentioned some examples including amphiceramides A and B, which have been extracted from the Caribbean sponge A. compressa. Noteworthy features of these glycolipids include a distinctive ∆6-phytosphingosine unit, and specifically for amphiceramide A, the presence of an unusual N-acetyl-β-glucosamine. Meanwhile, amphiceramide B stands out as the inaugural glycosphingolipid characterized by an allolactose residue that is β-linked to the ceramide component. In conclusion, these glycolipids from marine sources the authors mentioned are a diverse group of natural products with various biological activities, including antitumor and antiviral effects. This review focuses on their molecular diversity and synthesis, emphasizing how this helps in confirming their structures, understanding their biological roles, and designing new drugs.
Fig.1 Structures of the amphiceramides. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Amphiceramide can be studied to evaluate its absorption, distribution, metabolism, and excretion in the body, as well as its effectiveness and safety as a potential drug.
- Amphiceramide is researched for its interactions with other biomolecules to elucidate its biological roles and mechanisms.
- Amphiceramide can be used as a model compound to develop and refine new synthetic techniques and methods.
Advantages
- We obtain source specimens from high-quality marine organisms and maintain strict control over the preservation of these materials.
- We conduct a comprehensive process to ensure the effective production and purification of amphiceramides.
- Our analysis team utilizes diverse methods to perform accurate identification, characterization, and quantification of amphiceramide compounds.
Frequently Asked Questions
What types of amphiceramides can we produce?
What documentation is provided with the amphiceramide?
Amphiceramide is accompanied by a certificate of analysis (COA) detailing the product's purity, composition, and other relevant information. Furthermore, clients may also receive supplementary paperwork like safety data sheets (SDS) and technical data sheets.
At CD BioGlyco, our production process includes careful collection and purification methods to obtain high-quality samples. By isolating these compounds from their natural sources, we help clients study their unique properties and explore their potential applications in fields such as medicine research and biotechnology. If you want to understand the biological roles of these Marine Biomolecules and enhance your knowledge of marine biodiversity, contact us!
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
