Your Trusted Partner in Marine Ganglioside SJG Production
Gangliosides are an important class of glycolipid molecules in the nervous system, and they are involved in the onset and progression of a variety of neurological diseases. Notably, some of the gangliosides from marine organisms have stronger neurogenic activity than those from terrestrial organisms. SJG is a ganglioside from sea cucumbers with both SJG-1 and SJG-2 structures. At CD BioGlyco, we provide our clients with SJG-1 and SJG-2 production services based on state-of-the-art Marine Glycosphingolipid Production technology.
Extraction and Purification of SJG-1
- After cutting the body wall of the sea cucumber, SJG-1 is extracted from it using a mixture of CHCl3 and MeOH. After repeated extractions, all the extracts are combined and concentrated in a vacuum. Next, appropriate amounts of hexane, methanol, and acetone are added to it sequentially. The insoluble material is collected and dried to obtain the crude product of SJG-1 after stirring.
- We utilize reversed-phase column chromatography to purify SJG-1. In this process, silica gel is used as the stationary phase, and a solvent mixture of MeOH, CHCl3, and H2O is used as the mobile phase to purify SJG-1.
Extraction and Purification of SJG-2
- The extraction process of SJG-2 is similar to that of SJG-1. A certain ratio of trichloromethane and methanol is still used to extract from the body wall fragments of sea cucumbers.
- For the purification of SJG-2, we use reverse-phase column chromatography, ion exchange chromatography (IEC), and gel filtration chromatography (GFC).
First, the crude SJG-2 is placed in a silica gel column and eluted with methanol as eluent. After collecting the SJG-2-containing fraction, it is purified by an IEC and a GFC turn. Finally, the SJG-2 fraction is collected and dried in a vacuum to obtain pure SJG-2. In this process, we use thin-layer chromatography (TLC) to detect the purity of SJG-2, i.e., pure SJG-2 should have a spot on the TLC plate.
Characterization of SJG-1 and SJG-2
Both SJG-1 and SJG-2 have the appearance of an amorphous powder, and we offer characterization that includes, but is not limited to, the following:
- Structure: Nuclear magnetic resonance (NMR) and infrared (IR) spectroscopy are powerful methods for characterizing SJG-1 and SJG-2. Among them, IR can effectively characterize the hydroxyl group and amido group of SJG-1 and SJG-2.
- Relative molecular weight: The relative molecular weights of SJG-1 and SJG-2 are analyzed by fast-atom-bombardment mass spectrometry (FAMS).
- Purity: The purity of SJG-1 and SJG-2 is determined using high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS).
Publication Data
Technology: Solvent extraction method
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors describe the origin and bioactivity of the ganglioside SJG. SJG exists in two structures, SJG-1 and SJG-2, and both were isolated from the marine organism sea cucumber using an organic solvent. After bioactivity testing, it was found that SJG-1 and SJG-2 showed neurogenic activity against rat pheochromocytoma PC12 cells. Moreover, the activity of SJG-2 was higher than that of mammalian gangliosides.
Fig.1 Structure of SJG-1 and SJG-2. (Cheng-Sánchez & Sarabia, 2018)
Applications
- SJG promotes the growth and recovery of neurons and has a positive effect on the repair of damaged nerves. This allows it to be used in research on spinal cord injury and other nerve injuries.
- SJG is involved in regulating the growth, differentiation, and survival of neuronal cells, and has a role in promoting neuroplasticity, so it can be used to explore the mechanisms of neural development and nerve regeneration.
- SJG can be used in research on the treatment of various forms of acute brain injury, such as traumatic brain injury and ischemic brain injury, to reduce the extent of injury and promote recovery.
Advantages
- We have advanced Marine Biomolecule extraction, purification, and characterization technologies to confirm and analyze the physicochemical properties of SJG and ensure the obtaining of high-quality ganglioside SJG.
- We offer custom SJG production and development services based on client needs, including tailored SJG product specifications and purity requirements.
- In addition to SJG production services, we also provide detailed technical information and support, including SJG product applications and follow-up technical consulting services.
Frequently Asked Questions
What is the structural difference between SJG-1 and SJG-2?
Both SJG-1 and SJG-2 are overall composed of two parts, the glycan structure and the ceramide structure. Compared to SJG-1, SJG-2 has a more complex glycan structure, i.e., SJG-2 has a special hexasaccharide chain: NeuAc2→3(NeuAc2→4)→Gal1→8NeuAc2→3GalNAc1→3Gal1→4Glc.
What customized demands can clients put forward?
For the production of SJG, clients can put forward various customized demands, such as specific purity requirements, packaging forms, delivery times, etc. We will do our best to meet the needs of our clients.
CD BioGlyco provides first-class marine ganglioside SJG production solutions to our clients, and we are committed to producing high-quality marine ganglioside SJG. We have advanced and matured Marine Glycolipid Production processes, combined with a strong R&D team, to provide comprehensive support to our clients. Please feel free to contact us if you are interested in our services.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
