Superior Ganglioside Hp-s1 Production for Your Innovative Needs
Ganglioside Hp-s1 is a Marine Glycosphingolipid from the marine animal Hemicentrotus pulcherrimus or Diadema setosum with excellent neurogenic activity. CD BioGlyco has accumulated extensive experience in Marine Glycolipid Production and provides custom ganglioside Hp-s1 production solutions to our clients to help them understand and apply the biological functions of Hp-s1.
Extraction and Purification of Hp-s1
- We use a mixture of chloroform and methanol solvents to extract crude Hp-s1 from sea urchin spermatozoa. Next, the crude extract is dried in a vacuum and washed with phosphate buffer. Finally, the crude extract is dialyzed with distilled water and lyophilized to obtain a mixture of glycolipids including Hp-s1.
- We use column chromatography to purify Hp-s1 and monitor the chromatogram by high-performance thin-layer chromatography (HPTLC). In this procedure, the column is first eluted with chloroform, methanol, and water to remove neutral glycolipids. Then the column is eluted with a linear gradient of ammonium acetate and the target fraction, Hp-s1, is collected. We obtain the purity of Hp-s1 by HPTLC to ensure that it meets the client's research requirements.
Chemical Synthesis and Purification of Hp-s1
- The structure of ganglioside Hp-s1 contains two main parts, i.e., the glycan structure part and the ceramide structure part. Therefore, we chose glucose, sphingosine, and aminosugar as the raw materials, and Hp-s1 is prepared through a series of glycosylation and Staudinger reactions. Meanwhile, TLC is used to monitor the reaction process.
- Column chromatography is used to purify Hp-s1, in which silica gel is used as the stationary phase, and a mixture of methanol and chloroform, and a mixture of methanol, chloroform, and water are used as the mobile phases of the two stages, respectively.
Characterization of Hp-s1
- Structural characterization: Infrared (IR), and nuclear magnetic resonance (1H NMR, 13C NMR) are used to characterize the structure of Hp-s1.
- Molecular formula and molecular weight determination: High-resolution mass spectrometry (HRMS) is used to determine the molecular formula and molecular weight of Hp-s1.
Publication Data
Technology: Solvent extraction method, Western blot analysis and immunofluorescence evaluation
Journal: Marine Drugs
IF: 4.9
Published: 2020
Results: In this article, the authors describe the source and extraction method of ganglioside Hp-s1 and explore the anti-neuroinflammatory effects of Hp-s1 on lipopolysaccharide (LPS)-stimulated microglial cells. Ganglioside Hp-s1 was extracted from the sperm of sea urchins by solvent extraction method. After Western blot analysis and immunofluorescence evaluation, the authors found that Hp-s1 inhibited the expression of inducible nitric oxide synthase and cyclooxygenase-2, as well as proinflammatory cytokines. The findings suggest that Hp-s1 attenuates LPS-induced pro-inflammatory responses in microglia by down-regulating the MyD88-mediated NF-κB and JNK / p38 MAPK signaling pathways, suggesting that it is a novel anti-neuroinflammatory drug.
Fig.1 Hp-s1 structure (A) and effect on MG6 cell viability (B). (Shih, et al., 2020)
Applications
- Hp-s1 promotes the survival and regeneration of nerve cells and could be utilized to develop novel treatments for neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease.
- Due to its neuroprotective and anti-inflammatory properties, Hp-s1 can be used to develop high-end skin care products that promote the slowing down of skin aging and the repair of skin damage.
- Hp-s1 has the function of affecting the proliferation of cancer cells and inducing apoptosis, which can be used in the design and development of anti-cancer drugs.
Advantages
- We use advanced Marine Biomolecule Production technology to efficiently extract high-purity Hp-s1 from marine organisms.
- We have a strict quality control system to accurately test the indicators of Hp-s1 to ensure the safety and effectiveness of Hp-s1.
- According to the different needs of clients, we provide custom Hp-s1 production solutions, such as direct extraction solutions and chemical synthesis solutions.
Frequently Asked Questions
What is ganglioside Hp-s1? And what structural features does it have?
Ganglioside Hp-s1 is a complex glycolipid derived from marine organisms with a unique structure and function. Hp-s1 belongs to the ganglioside family, and its basic structure consists of a sphingolipid backbone and a polysaccharide chain, which usually includes multiple sialic acid subunits.
What are the biological activities of ganglioside Hp-s1?
Ganglioside Hp-s1 has a variety of biological activities such as:
- Neuroprotective activity: Ganglioside Hp-s1 has a significant protective effect on nerve cells, which can prevent and alleviate neurodegenerative diseases.
- Anti-inflammatory activity: Ganglioside Hp-s1 has significant anti-inflammatory properties, and plays an anti-inflammatory effect by regulating immune cell response and inhibiting the release of inflammatory mediators.
- Anti-tumor activity: Ganglioside Hp-s1 exhibits anti-tumor activity in certain cancer models and can regulate the growth and apoptosis of cancer cells.
CD BioGlyco has excellent marine biomolecule production technology and a professional team to provide custom marine ganglioside Hp-s1 production solutions to our clients. We focus on communication with our clients, providing detailed support and information sharing at every stage of the project to ensure the highest level of client satisfaction is achieved. Please feel free to contact us for your needs in marine ganglioside Hp-s1 production.
Reference
- Shih, J-H.; et al. Hp-s1 ganglioside suppresses proinflammatory responses by inhibiting MyD88-dependent NF-κB and JNK/p38 MAPK pathways in lipopolysaccharide-stimulated microglial cells. Marine Drugs. 2020, 18(10): 496.
