Leading Excellence in Marine Ganglioside GP-3 Production
Ganglioside belongs to the glycosphingolipid family and is commonly found in a variety of organisms. Among them, ganglioside GP-3 from sea cucumber has a unique structure with two internal sialic acid residues and three furanose residues, which has been widely noticed by scientists. CD BioGlyco has a professional team dedicated to research on Marine Biomolecule Production and has accumulated rich experience in Marine Glycosphingolipid Production, providing reliable ganglioside GP-3 production service to clients.
Extraction of Ganglioside GP-3
- GP-3 is extracted using a solvent mixture of methanol and chloroform in a certain proportion after chopping the whole body of the sea cucumber. After some time, the starfish residue is removed and the methanol and chloroform are removed in a vacuum to obtain a glycolipid-like mixture containing GP-3.
The glycolipid-like mixture is extracted using AcOEt/n-BuOH to remove the less polar glycolipids. Where the aqueous layer is then sequentially extracted using a n-BuOH and a mixture of methanol and chloroform and all solvents are removed in vacuo to give a crude GP-3.
- For the purification of GP-3, we perform a reversed-phase column chromatography. We use silica gel as the stationary phase of column chromatography, and different ratios of mixed solvents of methanol and chloroform as the eluent to purify GP-3. Meanwhile, the purified GP-3 showed a single spot on thin-layer chromatography (TLC).
Total Synthesis of Ganglioside GP-3
- In general, the synthesis of ganglioside consists of two main parts: the construction of the glycan structure and the selection of the ceramide structure.
Here, we perform the total synthesis of GP-3 by stereoselective and efficient glycosylation reactions. In this case, the construction of the glycan structure part is carried out by polymerization coupling operation. The glycan structure is used as a donor and conjugated with glucosylceramide acceptor to synthesize GP-3 in high yield.
- The GP-3 is purified by reversed-phase silica gel column chromatography, in which a mixture of methanol and chloroform in different ratios is selected as the eluent. The purified GP-3 is a white solid.
Characterization of Ganglioside GP-3
- Structure: NMR, IR, and MS are used to characterize the structure of GP-3.
- Molecular formula: The molecular formula of GP-3 is analyzed using elemental analysis and high-resolution mass spectrometry (HRMS).
Applications
- GP-3 plays an important protective role and promotes regeneration in neurons. It can be used to design and develop therapeutic programs for central nervous system-related diseases.
- GP-3 can be used to help scientists understand and probe cell signaling, cell membrane stability, and cell-cell interactions.
- Since GP-3 is abundantly expressed in the nervous system, targeting it can be used to develop drug delivery systems to improve the efficiency of drug delivery in the nervous system.
Advantages
- We have an experienced Marine Glycolipid Production team to provide comprehensive GP-3 production support to ensure that our client's research needs are met.
- We have advanced extraction and purification technologies to efficiently extract high-purity GP-3 from marine organisms and ensure the purity and consistency of GP-3 products.
- We master a wide range of GP-3 production technologies and provide custom solutions to meet specific application needs.
Frequently Asked Questions
What is marine ganglioside GP-3?
Marine ganglioside GP-3 is derived from sea cucumber and is a specific type of glycolipid with the appearance of a white solid powder. GP-3 has a specific structure with sialic acid residues and three furanose residues, which possess a variety of biomedical potential, such as neuroprotective and anti-tumor properties.
Do you provide technical support and after-sales service?
Yes, we provide comprehensive technical support and after-sales services, such as marine ganglioside GP-3 production and analysis technology consulting, and marine ganglioside GP-3-related questions and answers, to ensure that clients make full use of marine ganglioside GP-3.
CD BioGlyco has an experienced team of professionals, bringing together leading experts in the fields of biology, marine biology, and chemistry, to provide comprehensive marine ganglioside GP-3 production services. We flexibly adjust the production process according to the needs of our clients to ensure that each batch of product meets their specific requirements. If you have any questions or needs, please feel free to contact us, we will be happy to provide you with excellent service.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
