Purity and Precision in Cerebroside CE Production
Glycosphingolipids essential components of cell membranes in marine organisms, play a critical role in glycobiology. Marine sponge glycolipids have attracted significant interest due to their potential effects. CD BioGlyco provides diverse Marine Glycolipid Production Services by using advanced technologies. At CD BioGlyco, our researchers offer services for the production and analysis of Marine Glycosphingolipids, Marine Glycoglycerolipids, and Marine Atypical Glycolipids. Among them, cerebrosides, complex glycosphingolipids, are found in various biological sources, such as sea cucumbers. Many researchers are of interest because of their potential biological and pharmaceutical applications. In particular, we manufacture cerebrosides CE from the sea cucumber Cucumaria echinata. Our research team has the ability to extract many types of cerebrosides CE, including cerebroside CE-1-1, cerebroside CE-1-2, cerebroside CE-1-3, cerebroside CE-3-1, cerebroside CE-3-2, cerebroside CE-2b, cerebroside CE-2c and cerebroside CE-2d.
Extraction
We obtain the whole bodies of the sea cucumber C. echinata from the Ariake Sea. Then they are homogenized and extracted with methanol. The methanol extract is concentrated in vacuo to remove the solvent.
Purification
The residue is partitioned between water and chloroform. The organic layer is concentrated, yielding a less polar fraction. The less polar fraction is chromatographed on silica gel using solvents such as CHCl3 and MeOH. This process results in different fractions. We use normal-phase silica gel chromatography and reversed-phase chromatography to isolate cerebrosides CE.
Identification & Characterization
We identify cerebrosides CE through thin-layer chromatography, gas chromatography-mass spectrometry (GC-MS), and high-performance liquid chromatography (HPLC). Subsequently, we analyze cerebrosides CE obtained with optical rotation and nuclear magnetic resonance (NMR) spectroscopy to determine their structure.
Publication Data
DOI: org/10.3390/md21100539
Journal: Marine Drugs
Published: 2018
IF: 4.9
Results: In this review, authors explored the chemistry and biology of glycolipids and studied natural compounds that contain a glycosidic moiety connected to a lipid structure. Despite their diverse structural forms, glycolipids are generally classified into three primary categories: glycosphingolipids, glycoglycerolipids, and atypical glycolipids. This review underscores the remarkable structural and biological diversity inherent in marine-derived glycolipids, positioning them as prime candidates for chemical synthesis endeavors. The synthesis process is pivotal for validating molecular structures, elucidating biological functions, and developing analogues for pharmaceutical purposes. The authors mentioned that cerebrosides CE are extracted from the sea cucumber C. echinata, exhibiting toxicity in a brine shrimp lethality assay, with mortality rates ranging from 11% to 27%. The ultimate goal of this review is to stimulate interest in these natural substances and highlight the crucial role that chemical synthesis plays in advancing valuable bioactive compounds.
Applications
- Cerebrosides CE is useful for their potential to enhance neuronal growth and development due to their neuritogenic activity. This application is relevant for understanding how they might support neural regeneration and repair.
- Cerebrosides CE can be investigated for its ability to protect neurons from damage or degeneration, which can provide insights into their potential use in neuroprotection strategies, particularly in the context of neurodegenerative diseases.
- Cerebrosides CE can be used as candidates for its neuritogenic properties for developing new drugs aimed at promoting nerve growth and improving recovery from neurological injuries.
Advantages
- We utilize state-of-the-art methods to extract cerebrosides CE from sea cucumbers to ensure their high purity and efficiency.
- Our researchers employ both normal-phase silica gel chromatography and reversed-phase chromatography to achieve precise separation and isolation of cerebrosides CE.
- Our research team applies advanced analytical techniques including thin-layer chromatography, GC-MS, HPLC, optical rotation, and NMR spectroscopy for accurate identification and structural analysis.
Frequently Asked Questions
What are the biological activities of cerebrosides CE?
Cerebroside CE exhibits neuritogenic activity.
Can you provide custom Structure Modifications of cerebroside CE?
Yes, we can conduct the custom modification of cerebroside CE according to the client's specific requirements.
How long does it take to produce cerebroside CE?
Production time is determined by the complexity of the synthesis and the scale of production, so the process typically takes several weeks from beginning to end.
At CD BioGlyco, our researchers offer production services dedicated to extracting, purifying, and analyzing cerebrosides, with a specific focus on the compound known as cerebroside CE. For more information about our other Marine Biomolecule Production Services, including Marine Carbohydrates, Marine Glycoproteins, Marine Lectins, and Marine Glycosides, please don't hesitate to contact us.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
