Quality Plakoside Solutions Delivered
Plakoside is a Marine Glycosphingolipid with isoprenylated galactose and cyclopropane alkyl chains, which are highly immunosuppressive and non-cytotoxic. In addition, plakoside has potential therapeutic applications as a novel, non-cytotoxic immunosuppressant. CD BioGlyco has a team of long-term researchers dedicated to the Production of Marine Glycolipid, and we provide professional and reliable plakoside production services to our clients.
Extraction and Isolation of Plakoside
Plakoside is derived from the marine sponge Plakortis simplex, and we utilize an ethanol extraction method to extract plakoside from the sponge. First, the sponge is cut up and a suitable amount of ethanol is added to it. Next, the mixture of ethanol and sponge is centrifuged at high speed, and filtered and the filtrate is collected. A crude extract of plakoside is obtained after the concentration of the filtrate.
Purification of Plakoside
For the purification of plakoside, we use silica gel columns with dichloromethane-methanol as the eluent. After collecting the plakoside-containing eluent, it is concentrated and dried for processing to obtain plakoside in pure form.
We offer a multi-faceted plakoside characterization service to ensure the quality of plakoside.
- Structure: Mass spectrometry (MS), nuclear magnetic resonance (NMR), infrared (IR) and ultraviolet (UV) spectroscopy, etc.
- Purity: High-performance liquid chromatography (HPLC), gas chromatography (GC)
- Molecular weight: Gel permeation chromatography (GPC)
Bioactivity Analysis of Plakoside
Inducible nitric oxide synthase (iNOS) activity: Plakoside is diluted with ethanol and placed in a microtitre plate. The specific cells, interferon, and Griess reagent are added to it and incubated. After a while, the viability of the cells is measured by the Mossman method to get the iNOS activity profile of plakoside.
Publication Data
Technology: Organic solvent extraction method, NMR
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors describe the structural features of plakosides isolated from the sponge Plakortis simplex. Plakosides were isolated from the sponge by organic solvent extraction and their structure was determined by NMR. Plakosides' structure shows the presence of a cyclopropane ring in the lipid chain and 2-O isoprenylation in the carbohydrate portion. According to the bioactivity analysis, plakosides have a strong immunosuppressive activity. When T cells were stimulated with concanavalin A at test doses of 0.01-10 µg/mL, plakosides inhibited the proliferative response of lymph node cells.
Fig.1 Chemical structural formula of plakoside. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Plakoside has potent immunosuppressive activity and can be used in the development of immunosuppressive drugs and agents.
- Plakoside is shown to inhibit the proliferative response of lymph node cells, leading to applications in research on the body's immune response.
- A key role is played by plakoside in certain cancer types such as neuroblastoma and melanoma. Therefore, it can be used to develop new diagnostic markers and therapeutic targets for cancer.
Advantages
- Technical advantage: We have advanced Marine Biomolecule Production and purification technology, which enables us to produce high-quality plakoside efficiently and accurately.
- Quality control: A perfect quality control system has been established to ensure that the purity and stability of plakoside produced meet clients' requirements.
- Customized service: We customize the production of different types and specifications of plakoside according to the needs of our clients, providing flexible solutions.
Frequently Asked Questions
What is plakoside?
Plakoside is a glycosphingolipid from the marine sponge Plakortis simplex, whose structure features a lipid fraction containing cyclopropane alkyl chains and a carbohydrate fraction that produces 2-O-isoprenylation. It is a marine diosylceramides with no cytotoxicity and good immunosuppressive activity.
What are the purification methods for plakoside?
Purification of plakoside is a critical step in the production process of the compound, ensuring the purity and quality of the final product. Below are some common purification methods:
- Chromatography: Separation and purification of plakoside by the difference in retention time of plakoside and other impurities in the column.
- Ultrafiltration and microfiltration: Separation of plakoside and different-sized molecules using membranes with appropriate pore sizes, which can be used in the initial purification step.
- Centrifugation: High-speed centrifugation can be used to separate components of a suspension, effectively removing insoluble impurities and enriching plakoside.
CD BioGlyco utilizes modern biological and chemical production processes to provide high-quality and reliable plakoside production services to our clients. Please feel free to contact us if you are interested in our plakoside production services.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
