Crafting Excellence in Clarhammoside Solutions
Clarhammoside from the marine sponge Agelas clathrodes is a specialized glycosphingolipid that has attracted the attention of scientists due to its novel structure with a terminal rhamnose unit. CD BioGlyco has a team of experts dedicated to Marine Glycosphingolipid research to provide trusted clarhammoside services to our clients.
Extraction of Clarhammoside
The sponge is pulverized and a specific amount of methanol and chloroform mixture is added to it to extract the clarhammoside. After some time, the sponge residue is filtered and removed. At this point, the methanol and chloroform mixture containing clarhammoside is partitioned between water and BuOH, and the organic layer is collected. After concentrating on the organic layer, a crude product of clarhammoside will be obtained.
Purification of Clarhammoside
Initial purification of crude clarhammoside on silica gel and SiO2 columns led to a mixture of clarhammoside and other glycolipids. The mixture of clarhammoside and other glycolipids is acetylated, the other glycolipids are removed by high-performance liquid chromatography (HPLC), and the fraction containing acetylated clarhammoside is collected. Finally, the acetylated clarhammoside is deacetylated to obtain a pure product of clarhammoside.
Analysis of Clarhammoside
- Structural analysis: Electrospray ionization mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR) are used to analyze the structure and conformation of clarhammoside.
- Molecular weight analysis: The molecular weight of clarhammoside is analyzed by MS coupled with gel permeation chromatography (GPC).
- Purity analysis: The purity of produced clarhammoside is analyzed using HPLC and gas chromatography (GC).
- Decomposition mechanism detection: Use circular dichroism (CD) spectrum to track the structural changes of clarhammoside when it decomposes, and then deduce the decomposition mechanism of clarhammoside.
On the other hand, our professional Marine Glycolipid R&D team also provides the chemical synthesis solution of clarhammoside for our clients.
Since the structure of the glycan chain of clarhammoside is characterized by the fact that the inner galactose is connected to ceramide via an α-glycosidic bond, and the 2-position of this glycan is connected to another galactose via an α-glycosidic bond, we synthesize clarhammoside by dividing it into four blocks and synthesizing them individually, and by connecting the four blocks in sequence via the stepwise extension method. For chemically synthesized clarhammoside, we similarly use the above analysis method to analyze the properties of clarhammoside.
Publication Data
Technology: Solvent extraction, NMR, ESI-MS
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors show the extraction and isolation of marine natural glycosphingolipid clarhammoside from sponges. The extraction and isolation of clarhammoside was carried out by organic solvent extraction and its purification was carried out by chromatographic techniques. Finally, the structure of clarhammoside was characterized by NMR and ESI-MS. Clarhammoside is an α-galactosyl sphingolipid with an l-rhamnose unit at the end of the sugar chain, and two consecutive 1,2-cis-α-d-galactopyranoside bonds are also present.
Fig.1 The structure of clarhammoside. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Clarhammoside stimulates NKT cells to exert its anti-tumor effect, so clarhammoside can be used in the design and development of anti-tumor drugs.
- Clarhammoside has special immunomodulatory activity, which can be used in the research of immune diseases.
- Clarhammoside is involved in cell growth regulation and messaging processes and can be used in the design and development of glycolipid drugs that block cell adhesion and immunomodulation.
Advantages
- We have mastered advanced Marine Biomolecule Production technology to realize large-scale and high-yield clarhammoside production.
- We have constructed a multidisciplinary team of experts, including biologists and chemists, to provide custom clarhammoside production solutions to our clients, and provide full support for their research.
- We not only provide clients with custom clarhammoside production solutions but also provide detailed structure analysis and property analysis reports to help clients apply clarhammoside.
Frequently Asked Questions
What are the structural features of clarhammoside?
The structure of clarhammoside is α-L-Rhap-(1→3)-β-D-NHAcGalp-(1→6)-α-Galp-(1→2)-α-Galp-ceramide. The structure of the glycan chain of this compound is characterized by the connection of the inner galactose to ceramide via an α-glycosidic bond, and the 2-position of this glycan is connected to another galactose via an α-glycosidic bond.
What are the biological activities of clarhammoside?
Clarhammoside is an important biologically active molecule that contains a variety of biological activities. For example, clarhammoside is involved in neuronal and glial cell recognition and adhesion, and the regulation of intercellular signaling and cellular communication. Clarhammoside also plays an important role in the immune system, participating in the regulation of immune response and pathogen recognition by interacting with receptors on the surface of immune cells.
With excellent technical strength and a professional team, CD BioGlyco provides personalized clarhammoside production services. We are committed to ensuring high-quality clarhammoside products and providing the best solutions for our clients. Our production system strictly controls every step of the process to ensure the purity and stability of our products. Please feel free to contact us if you have any needs or questions.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
