Unlocking the Ocean's Potential with Premier Marine Ganglioside CEG
Gangliosides belong to the glycosphingolipid family, which is mainly composed of hydrophilic oligosaccharide chains and hydrophobic amides. Marine organisms are an important source of gangliosides. CD BioGlyco provides comprehensive marine gangliosides production service to clients with first-class Marine Biomolecule Production technology and rich research experience.
Here, we offer marine ganglioside CEG extraction, purification, and characterization services as shown below.
Ganglioside CEG Extraction Service
We use organic solvents to extract CEG from sea cucumbers. Firstly, the sea cucumber is chopped and then extracted using trichloromethane and methanol. To obtain a high yield of CEG, several extraction operations are required. After that, all extracts are combined and concentrated. Next, the obtained concentrate is placed in acetone. Among them, the part that is not dissolved in acetone belongs to the polar lipid fraction, which is a mixture of glycolipids containing CEG.
Ganglioside CEG Purification Service
We use continuous column chromatography to purify CEG, in which all column chromatography uses silica gel as a stationary phase. At the same time, mixtures of trichloromethane, methanol, and water in different ratios are used as mobile phases.
The purified CEG is examined by thin-layer chromatography (TLC), i.e., the pure CEG has only one spot on the TLC plate.
Ganglioside CEG Characterization Services
We provide characterization services to help our clients understand the properties of CEGs and to make full use of CEGs, which include, but are not limited to, the following:
- Structural characterization: NMR, IR spectroscopy, and fast atom bombardment mass spectrometry (FAMS) are used to analyze the structure of the CEG, e.g. the number of atoms, the types and positions of functional groups, etc.
- Purity analysis: TLC and HPLC are used to determine the purity of CEG.
Analysis of Neuritogenic Activity of Ganglioside CEG
The neuritogenic activity of CEG is detected using PC12 cells. We culture PC12 cells and CEG together and set up a control group with/without nerve growth factor (NGF). After a while, the morphological changes of the cells, i.e. the presence or absence of neurite formation, are observed using a light microscope.
Publication Data
Technology: Solvent extraction and column chromatography
Journal: Marine Drugs
IF: 4.9
Published: 2018
Results: In this article, the authors demonstrate the origin and neurogenic activity of gangliosides CEG-3, CEG-4, CEG-5, CEG-6, CEG-8, and CEG-9. Gangliosides CEG-3, CEG-4, CEG-5, CEG-6, CEG-8, and CEG-9 were extracted and isolated using mixed solvents from the sea cucumber Cucumaria echinata and purification was accomplished using column chromatography. In addition, after bioactivity analysis, the authors found that these compounds exhibited neural nerve growth activity against rat pheochromocytoma cell line PC-12 in the presence of NGF.
Fig.1 The structure of marine gangliosides CEGs. (Cheng-Sánchez & Sarabia, 2018)
Applications
- Gangliosides CEG has the potential to promote the growth of nerve cells, so it can be used to develop drugs to treat diseases related to the nervous system.
- Gangliosides CEG can be used to design and develop markers in the nervous system to help researchers understand the mechanisms of neurological diseases.
- Marine gangliosides can inhibit the growth and spread of certain cancer cells and have anti-cancer potential. Therefore, marine gangliosides CEG can be used in research on the treatment and prevention of cancer.
Advantages
- We utilize modern Marine Glycolipid Production technology to efficiently extract and isolate gangliosides CEG from marine organisms.
- We have a professional Marine Glycosphingolipid research team, which can carry out in-depth research and development to provide custom gangliosides CEG products to our clients.
- We provide professional client service and technical support to help our clients solve their needs and problems in the application of gangliosides CEG products.
Frequently Asked Questions
What is the structure of gangliosides CEG?
Gangliosides CEG consists of a complex oligosaccharide chain and a lipid fraction. The oligosaccharide chain is connected to the lipid fraction by a glycosidic bond. The lipid fraction consists of a structure formed by sphingosine and fatty acids, which are the hydrophobic part of gangliosides and help anchor them in the cell membrane.
What are the biological activities of gangliosides CEG from marine organisms?
Marine gangliosides CEG has a wide range of biological activities that make it important in a variety of fields. Some of the common biological activities include neuroprotective, anti-inflammatory, and anti-cancer activities.
CD BioGlyco has leading marine biomolecule extraction and purification technologies to produce marine gangliosides CEG with high efficiency and quality. Our dedicated team provides tailor-made solutions for the production of gangliosides CEG and offers comprehensive technical support such as product development, process optimization, and analytical testing. Please feel free to contact us for any request or inquiry.
Reference
- Cheng-Sánchez, I.; Sarabia, F. Chemistry and biology of bioactive glycolipids of marine origin. Marine Drugs. 2018, 16(9): 294.
